Energy Dependent Bivalent Cation Translocation in Rat Liver Mitochondria

Translocation to rat liver mitochondria of phosphatidate phosphohydrolase.

When a particle-free supernatant fraction from rat liver was incubated at 37 degrees C with mitochondria and oleate, some of the enzyme phosphatidate phosphohydrolase (PAP), initially present in the particle-free supernatant, was recovered, after the incubation, bound to mitochondria. This translocation of PAP from cytosol to mitochondria was stimulated by oleate or palmitate in a similar fashi...

Steady state kinetics of energy-dependent Ca2+ uptake in rat liver mitochondria.

The divalent cation ionophore A23187 has been used to examine the steady state kinetics of energy-dependent Ca2+ uptake with /3-hydroxybutyrate as oxidizable substrate, and these results have been compared to the kinetic parameters obtained with succinate as the available energy source. The kinetics are sigmoidal with the free Ca*+ concentration at half-maximal respiratory stimulation (K,,,,) e...

Food restriction affects energy metabolism in rat liver mitochondria.

To examine the effect of 50% food restriction over a period of 3 days on mitochondrial energy metabolism, liver mitochondria were isolated from ad libitum and food-restricted rats. Mitochondrial enzyme activities and oxygen consumption were assessed spectrophotometrically and polarographically. With regard to body weight loss (-5%), food restriction decreased the liver to body mass ratio by 7%....

Steady State Kinetics of Energy-dependent Ca2+ Uptake in Rat Liver Mitochondria*

The divalent cation ionophore A23187 has been used to examine the steady state kinetics of energy-dependent Ca2+ uptake with /3-hydroxybutyrate as oxidizable substrate, and these results have been compared to the kinetic parameters obtained with succinate as the available energy source. The kinetics are sigmoidal with the free Ca*+ concentration at half-maximal respiratory stimulation (K,,,,) e...

Energy-linked Incorporation of Diphosphopyridine Nucleotide into Rat-liver Mitochondria.